Mushrooms, Biomarker Tests, and Cancer: Evidence-Based Connections

Last Updated on October 30, 2025

A growing body of high-quality research links mushroom consumption and mushroom-derived compounds with positive influences on cancer biomarkers and potential cancer outcomes. This article reviews the state of the science on the topic, summarising laboratory, animal, and epidemiological findings, and explains the role of biomarker testing in modern cancer medicine.

How Mushrooms Influence Cancer Biology

Mushrooms have long been a part of complementary medicine, with recent scientific focus on isolating their bioactive molecules for therapeutic potential. Key effects relevant to cancer include:

  • Immune Modulation: Certain mushrooms, such as Polyporus linteus and Trametes versicolor (turkey tail), have been shown to enhance T cell numbers, increase secretion of immune factors like IL-12, IFN-γ, and TNF-α, and stimulate natural killer (NK) cell activity. These immunological shifts are thought to support anti-tumor defence.
  • Direct Tumor Effects: Extracts from mushrooms like Agaricus bisporus (white button), Lentinus crinitus, and Ganoderma species suppress cancer cell proliferation, induce apoptosis (programmed cell death), and inhibit key growth pathways in animal models and cell cultures.
  • Disruption of Cancer Pathways: Mushroom compounds influence crucial molecular pathways (e.g., PI3K/AKT, MAPK, p53, WNT, NOTCH), restoring normal metabolic and apoptotic signaling and interfering with cancer cell survival.
  • Influence on Metabolic Biomarkers: Compounds such as neoalbaconol, panepoxydone, and conjugated linoleic acid alter lipid and carbohydrate metabolism, normalize the TCA cycle, and modulate inflammatory biomarker production (e.g., COX-2, prostaglandin E2).

Evidence from Proteomic and Clinical Animal Studies

Large-scale proteomic studies using advanced animal models show that blends of medicinal mushrooms can:

  • Increase survival in colon cancer models (sometimes alongside standard chemotherapy).
  • Change expression of biomarkers for ribosomal biogenesis (RPS3), translation processes (RPL14), unfolded protein response (DNAJC3), lipid metabolism (ACOT7), and the tricarboxylic acid cycle (FH).
  • Suppress chronic inflammatory biomarkers and angiogenesis (e.g., COX-2 inhibition, VEGF reduction) while inducing tumor apoptosis.

Population Evidence and Epidemiology

Systematic reviews and meta-analyses of observational studies indicate:

  • Higher mushroom consumption—about two standard mushrooms per day—is associated with a statistically significant decrease in total cancer risk, especially for breast and gastric cancers.
  • Not all studies find associations for every cancer type; individual risk factors, lifestyle, and genetics also play key roles.

Table: Summary of Mushroom-Cancer Biomarker Evidence

Mushroom SpeciesMain BioactivesCancer/Marker StudiedKey Findings/Effects
Trametes versicolorPSP, polysaccharidesBreast, prostate, gastric, colonImmune activation, reduced proliferation
Agaricus bisporusConjugated linoleic acidBreast, prostate, colonAromatase inhibition, induced apoptosis
Ganoderma speciesGL22, triterpenesLiver, general cancer pathwaysLipid metabolism alteration, cell death
Grifola frondosaBeta-glucansInflammation, general cancerCOX-2 inhibition, immune modulation

Why Biomarker Tests Matter

A biomarker test by companies like Superpower, is a laboratory assessment that analyses blood, tissue, or other samples to detect specific genes, proteins, or molecular markers. These tests reveal information about cancer presence, type, likely progression, and best therapeutic approaches. In cancer research and clinical practice, biomarker tests are used to:

  • Track how mushrooms or other interventions affect tumor and immune markers.
  • Guide patient treatment selection and measure therapy effectiveness.

Practical Guidance

Compelling evidence shows that mushrooms—and particularly their isolated molecules—can influence key cancer-associated biomarkers, with effects seen in immune function, cell proliferation, tumor apoptosis, and metabolic regulation. While population data indicate potential cancer risk reduction from higher mushroom intake, most clinical proof stems from animal and laboratory findings. Human biomarker tests should continue to guide cancer care, integrating new findings on dietary and therapeutic interventions.

If you are interested in how food or supplements influence cancer or want to monitor your own or a patient’s biomarker response, discuss biomarker test options with a healthcare provider. As research grows, biomarker testing remains the gold standard for assessing interventions, including mushrooms, in cancer prevention and treatment strategies.

References

Dowaraka-Persad B, Neergheen VS. Mushroom-Derived Compounds as Metabolic Modulators in Cancer. Molecules. 2023. [PMC9920867]​

Jakopovic M, San DM, et al. Proteomic study of medicinal mushroom extracts reveals antitumor mechanisms in an advanced colon cancer animal model via ribosomal biogenesis, translation, and metabolic pathways. Front Pharmacol. 2024. [Frontiers in Pharmacology]​

Patel S, Goyal A. Recent developments in mushrooms as anti-cancer therapeutics: a review. 3 Biotech. 2012;2(1):1–15. [PMC3339609]​

Nandi S, et al. A review for cancer treatment with mushroom metabolites: From traditional use to clinical trial evidence. Life Sciences. 2024. [ScienceDirect]​

Venturella G, Ferraro V, Papa G, et al. Medicinal Mushrooms: Bioactive Compounds, Use, and Clinical Studies. Int J Mol Sci. 2021. [PMC7826851]​

World Cancer Research Fund. Should we eat mushrooms to prevent cancer? 2025.​

National Foundation for Cancer Research. Mushrooms for cancer prevention and mental wellbeing. 2025.​

Cleveland Clinic. Biomarker Testing for Cancer. 2025.​

National Cancer Institute. Definition of biomarker testing. 2025.​

American Cancer Society. Biomarker Tests and Cancer Treatment. 2022.​

Author

  • World Mushroom Society is a collective of medicinal mushroom advocates and contributors. Our team of authors includes backgrounds in environmental science, neuroscience, bio-molecular technology, agronomy and general health.

World Mushroom Society is a collective of fungi enthusiasts and health advocates, sharing information, research, studies, and identifying top producers of high quality medicinal mushroom supplements.

DISCLAIMER

DISCLAIMER
The information on this website is not intended to diagnose, treat, cure or prevent any disease. All information found here is not meant as a substitute for, or alternative to, information from your doctor for ongoing medical treatment you currently receive. If unsure, please consult with your doctor before using medicinal mushrooms. Any content related to cancer should not be considered as prescriptive medical advice and should not be a substitute for any cancer treatment, unless advised by your doctor first. The efficacy of these products has not been confirmed by TGA and FDA-approved research. If you are pregnant or on prescription drugs that thin the blood, consult with your medical professional before using medicinal mushrooms.
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